Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

View All Documentation

MAC4L

Sigma-Aldrich

MetaPolyzyme

lyophilized powder

Sinónimos:

Multilytic Enzyme Mix

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

technique(s)

DNA extraction: suitable

suitability

suitable for microbiology

application(s)

microbiology

shipped in

wet ice

storage temp.

−20°C

General description

Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.

Application

MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG). Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.

Biochem/physiol Actions

Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.

Components

The enzymes in MetaPolyzme are:
  • Achromopeptidase
  • Chitinase
  • Lyticase
  • Lysostaphin
  • Lysozyme
  • Mutanolysin

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Erratum to: The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium Inaugural Meeting Report.
Microbiome, 4(1), 45-45 (2016-08-20)
Maria A Sierra et al.
Environmental microbiome, 17(1), 60-60 (2022-12-22)
Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional
Nezar Noor Al-Hebshi et al.
Journal of oral microbiology, 11(1), 1557986-1557986 (2019-01-24)
Studies of the microbiome associated with dental caries have largely relied on 16S rRNA sequence analysis, which is associated with PCR biases, low taxonomic resolution, and inability to accurately study functions. Here, we employed whole metagenome shotgun sequencing, coupled with
Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome.
Maghini DG et al.
Nature Protocols, 16, 458-471 (2021)
Scott Tighe et al.
Journal of biomolecular techniques : JBT, 28(1), 31-39 (2017-03-25)
The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques.
Ver todos los artículos relacionados

Artículos

Lysing Enzymes

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

Lysing Enzymes

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

Lysing Enzymes

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

Lysing Enzymes

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico