L6397
Concanavalin A from Canavalia ensiformis (Jack bean)
peroxidase conjugate, lyophilized powder
Sinónimos:
ConA
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
Canavalia ensiformis (jack bean)
Nivel de calidad
conjugado
peroxidase conjugate
Formulario
lyophilized powder
técnicas
blood typing: suitable
fractionation: suitable
impurezas
carbohydrate, essentially free
temp. de almacenamiento
−20°C
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Aplicación
Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Acciones bioquímicas o fisiológicas
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Envase
Package size based on protein content
Definición de unidad
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Forma física
Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese
Nota de preparación
Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane,[1] which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.
Nota de análisis
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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