This product is not tested for solubility. Various sources suggest that this material may be reconstituted in water or aqueous buffers at 5 mg/ml. This information has not been validated.
L3908
ββ-Lactoglobulina from bovine milk
≥90% (PAGE), lyophilized powder
Sinónimos:
βLg, BLG, Bos d 5, beta-lactoglobulin
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About This Item
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Productos recomendados
biological source
bovine milk
Quality Level
assay
≥90% (PAGE)
form
lyophilized powder
technique(s)
ELISA: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... LGB(280838)
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General description
La leche de las vacas lecheras contiene la proteína β-lactoglobulina (BLG). Aparece de manera natural en una serie de variantes genéticas, y las variantes bovinas más prevalentes son la BLG A y la BLG B.[1]
Application
βLa β-lactoglobulina se utilizó en el análisis citológico para el diagnóstico de la hipersensibilidad alimentaria en pacientes con síndrome de intestino irritable.[2]
Biochem/physiol Actions
Miembro de la familia de las lipocalinas, la βLg es una proteína pequeña de 162 aminoácidos con una masa molecular de ∼18 400 Da. Consta de un barril β de ocho cadenas (cadenas A-H) seguidas de una hélice α de tres vueltas y una cadena β final (cadena I) que forma parte de la interfaz de dimerización.[3]
Other Notes
Contiene β-lactoglobulinas A y B, que pueden aislarse mediante cromatografía.
Quality
Puede no contener proteína de unión al folato; no recomendada para análisis de folatos.
Preparation Note
Purificada por cromatografía
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Sirpa Jylhä et al.
Journal of immunological methods, 350(1-2), 63-70 (2009-08-04)
Cow's milk allergy (CMA) is a common food allergy, especially among infants and young children. Approximately 85% of milk-allergic children outgrow their allergy by the age of three but the remaining 15% remain allergic. Bovine beta-lactoglobulin (BLG) is one of
Lena Wartosch et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(12), 4056-4068 (2009-08-08)
Mutations in either ClC-7, a late endosomal/lysosomal member of the CLC family of chloride channels and transporters, or in its beta-subunit Ostm1 cause osteopetrosis and lysosomal storage disease in mice and humans. The severe phenotype of mice globally deleted for
Tilman Barz et al.
Journal of chromatography. A, 1217(26), 4267-4277 (2010-05-07)
In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the
Leonor Pérez-Fuentes et al.
Soft matter, 13(6), 1120-1131 (2017-01-18)
In this study we have investigated how different proteins interact with big organic ions. Two ions that are similar in size and chemical structure (Ph
Özge Dönmez et al.
Food & function, 11(7), 6038-6053 (2020-06-20)
The effect of binding of flavonoids, (-)-epigallocatechin-3-gallate (EGCG) and green tea extract (GTE), to beta-lactoglobulin (β-Lg) and micellar casein (micellar casein isolate, MCI) on protein digestibility was investigated. β-Lg resisted digestion by pepsin, but in the presence of EGCG the
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What is the preferred buffer to dilute the product in? Or is it deionized water with or without protease inhibitors? (Application: SDS-PAGE gels and western blot)
1 answer-
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does this product have other components such as sucrose, trehalose or salts?
1 answer-
This product is prepared from skimmed milk. Lactoglobulins are precipitated and further purified chromatographically. The final dialysis process depletes most residual salts. The minimum purity of this product is 90%. While sugars such as trehalose and sucrose would not be expected, the impurities of this product have not been identified.
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How is the solubility of the product in water?
1 answer-
This product is not tested for solubility. Various sources suggest that this material may be reconstituted in water or aqueous buffers at 5 mg/ml. This information has not been validated.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product L3908, β-Lactoglobulin from bovine milk?
1 answer-
Product L3908, β-Lactoglobulin from bovine milk, exists as a dimer of two peptides. Peptide A = 18,367, B = 18,281; the monomer is approximately 36,600.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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