L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• Sinónimos: Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• Número de CAS: 9001-60-9
• Comisión internacional de enzimas: 1.1.1.27 (BRENDA, IUBMB)
• Número CE: 232-617-8
• Número MDL: MFCD00131466
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine heart
• Nivel de calidad: 200
• tpo: Type XVII
• Ensayo: 5.0—15.0 mg protein/mL (biuret)
• Formulario: buffered aqueous glycerol solution
• actividad específica: ≥400 units/mg protein
• mol peso: 140 kDa
• fabricante / nombre comercial: Sigma-Aldrich
• técnicas: cell based assay: suitable
• impurezas: <10 μg/mg protein free ammonium
• color: white
• pH: 7.5
• trazas de catión: NH₄⁺: ≤10 μg/mg protein
• Nº de acceso UniProt: P19858; Q5E9B1
• aplicaciones: life science and biopharma
• actividad extraña: glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%; pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: cow ... LDHA(281274) , LDHB(281275)

Descripción

Descripción general

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

Aplicación

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

Acciones bioquímicas o fisiológicas

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.

Definición de unidad

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forma física

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Nota de análisis

Protein determined by biuret

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1