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H1136

Sigma-Aldrich

Hyaluronidase from Streptomyces hyalurolyticus

Sinónimos:

Hyaluronate Lyase from Streptomyces hyalurolyticus

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About This Item

Número de CAS:
37259-53-3
Comisión internacional de enzimas:
4.2.2.1 (BRENDA, IUBMB)
Número CE:
253-430-8
Número MDL:
MFCD00131347
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

Streptomyces sp. (S. hyalurolyticus)

Nivel de calidad

200

conjugado

conjugate (Glucosaminoglycan)

Formulario

film (Clear)

envase

ampule of 300 ×  units

temp. de almacenamiento

−20°C

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Descripción general

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Aplicación

Hyaluronidase from Streptomyces hyalurolyticus has been used:
  • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
  • as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Acciones bioquímicas o fisiológicas

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Definición de unidad

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000419723
0000408483
0000408483
0000419723
0000405614

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Crystal structure of hyaluronidase, a major allergen of bee venom
Markovic H, et al.
Structure, 8(10), 1025-1035 (2000)
Connor C Gains et al.
Frontiers in bioengineering and biotechnology, 8, 700-700 (2020-08-09)
The Achilles tendon (AT) is comprised of three distinct sub-tendons bound together by the inter-subtendon matrix (ISTM). The interactions between sub-tendons will have important implications for AT function. The aim of this study was to investigate the extent to which
Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
Ko K, et al.
Nature Protocols, 5(5), 921-921 (2010)
Swathi Balaji et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 868-881 (2016-12-03)
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that
Wen Min Lau et al.
Cancer research, 74(9), 2630-2641 (2014-03-13)
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In
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Protocolos

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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