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H0262

Sigma-Aldrich

HAT Media Supplement (50×) Hybri-Max™

lyophilized powder, suitable for hybridoma, BioXtra

Sinónimos:

HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nombre del producto

HAT Media Supplement (50×) Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

Nivel de calidad

200

esterilidad

γ-irradiated

Línea del producto

BioXtra

Formulario

lyophilized powder

técnicas

cell culture | hybridoma: suitable

impurezas

endotoxin, tested

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Aplicación

HAT Media Supplement (50×) Hybri-Max™ has been used:
  • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
  • in the generation of mAbs (monoclonal antibodies)
  • in the luciferase-based bioassay (LUC) assay

Reconstitución

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Información legal

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H302,H360FD

Clasificaciones de peligro

Acute Tox. 4 Oral - Repr. 1B

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000359628
0000359628
0000330921
0000334051
0000384631

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Geoffrey A Mueller et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 1999-2007 (2020-09-11)
IgE Abs drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils. If the IgE binding sites on the allergens could be identified, it may be useful for creating new forms of immunotherapy. However, direct knowledge
J H Connolly et al.
Journal of anatomy, 195 ( Pt 2), 161-171 (1999-10-21)
The gross and histological appearance and the distribution of T and B lymphocytes and plasma cells are described for lymphoid tissues obtained from 15 platypuses. The spleen was bilobed and surrounded by a thick capsule of collagen, elastic fibres and
Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta
Hermanrud C, et al.
Journal of Immunological Methods, 1-9 (2016)
The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of Immunology, jimmunol-j0804281 (2009)
Youngkyun Kim et al.
Clinical & translational immunology, 4(11), e51-e51 (2015-12-19)
This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the
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