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Key Documents

GEPA17004

Sigma-Aldrich

Cy®7 Bis NHS Ester

Cytiva PA17004, pack of 10 mg

Sinónimos:

N-hydroxysuccinimide ester, NHS Ester

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

packaging

pack of 10 mg

manufacturer/tradename

Cytiva PA17004

storage condition

protect from light (Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.)

color

Purple

storage temp.

2-8°C

General description

Cy®7 Bis NHS Ester or N-hydroxysuccinimide ester is a commercially available fluorescent label. The two reactive groups present on each dye molecule enable the labeling of the molecules. NHS esters react with nucleophiles to release the NHS or sulfo-NHS group to create stable amide and imide bonds.

Application

Cy®7 Bis NHS Ester is used for HTS (High Throughput Screening) experiments.

It is a preferred fluorescent label for site-specific RNA labeling. The spectrophotometric assays using NHS esters are used to measure the active esters on carriers and the subsequent interaction with amines during peptide synthesis.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

CyDye:
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva UK Limited.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva UK Limited.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of General Electric Company or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact LSlicensing@ge.com.

If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
Cy is a registered trademark of Cytiva

pictograms

Health hazard

signalword

Warning

hcodes

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A spectrophotometric assay for soluble and immobilized N-hydroxysuccinimide esters.
T Miron et al.
Analytical biochemistry, 126(2), 433-435 (1982-11-01)
Stefan Kalkhof et al.
Analytical and bioanalytical chemistry, 392(1-2), 305-312 (2008-08-30)
In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized
Mengyang Li
Bioengineered, 11(1), 599-606 (2020-05-26)
Site-specific fluorescent labeling of RNA is crucial for obtaining the structural and dynamic information of RNAs by fluorescence techniques. Post-synthetic modification of RNA based on N-hydroxysuccinimide (NHS) coupling reaction is an economic, efficient and simple strategy to introduce fluorophore to

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