GE28-9782-45

HiScreen^™, DEAE Fast Flow

Cytiva 28-9782-45

• Código UNSPSC: 47101511
• NACRES: NA.56

Propiedades

• ligand: diethylaminoethyl
• descripción: Ion Exchanger Type (value)
• Formulario: prepacked column
• reticulante: cross-linked
• envase: pkg of 1 ea
• fabricante / nombre comercial: Cytiva 28-9782-45
• condiciones de almacenamiento: (20% Ethanol)
• Parámetros: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• técnicas: liquid chromatography (LC): suitable
• lecho tamaño: 7.7 mm × 100 mm
• lecho volumen: 4.7 mL
• columna D.I.: 7.7 mm
• Matriz: 6% cross-linked agarose
• grupo activo de la matriz: N⁺(C₂H₅)₂H functional group
• tamaño de partícula: 45-165 μm
• diámetro promedio: 90 μm
• cleaning: 2-14
• Intervalo de trabajo: 2-12
• capacidad: binding capacity(~ 110 mg HAS/mL resin)
• idoneidad: suitable for bioprocess medium
• técnica de separación: anion exchange

Descripción

Descripción general

HiScreen^™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Aplicación

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen^™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Características y beneficios

• DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose^™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
• Prepacked, ready-to-use columns for convenient process development optimization.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids