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Key Documents

GE17-1279-03

rProtein A Sepharose Fast Flow

Cytiva 17-1279-03, pack of 200 mL

Sinónimos:

antibody affinity medium, antibody purification resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-1279-03

storage condition

(20% Ehtanol)

matrix

4% cross-linked agarose

particle size

60-165 μm

average diameter

90 μm (d50v)

cleaning in place

2-11

working range

3-10

capacity

~50 mg binding capacity (human lgG/ml medium)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Maida Romera-Branchat et al.
Cell reports, 31(9), 107717-107717 (2020-06-04)
Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genus Arabidopsis contain two of these bZIPs, FD and FDP, which we show have largely complementary expression
Lawrence T Wang et al.
Cell reports, 38(7), 110367-110367 (2022-02-17)
L9 is a potent human monoclonal antibody (mAb) that preferentially binds two adjacent NVDP minor repeats and cross-reacts with NANP major repeats of the Plasmodium falciparum circumsporozoite protein (PfCSP) on malaria-infective sporozoites. Understanding this mAb's ontogeny and mechanisms of binding
Lawrence T Wang et al.
Immunity, 53(4), 733-744 (2020-09-19)
Discovering potent human monoclonal antibodies (mAbs) targeting the Plasmodium falciparum circumsporozoite protein (PfCSP) on sporozoites (SPZ) and elucidating their mechanisms of neutralization will facilitate translation for passive prophylaxis and aid next-generation vaccine development. Here, we isolated a neutralizing human mAb

Artículos

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Protocolos

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

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