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GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

Sinónimos:

affinity chromatography resin, GST-tagged proteins purification

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

envase

pack of 100 mL

fabricante / nombre comercial

Cytiva 17-0756-05

Matriz

4% agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning in place

4-13

Intervalo de trabajo

4-13

capacidad

>25 mg binding capacity (horse liver GST/ml medium)

Descripción general

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Características y beneficios

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Artículos

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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Protocolos

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

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