G5642

Monosialoganglioside GM₃ from canine blood

≥98%, lyophilized powder

• Número de CAS: 54827-14-4
• MDL number: MFCD00131139
• UNSPSC Code: 12352200
• NACRES: NA.77

Propiedades

• Quality Level: 200
• assay: ≥98%
• form: lyophilized powder
• solubility: chloroform/methanol/water (9.5:9.5:1): 20 mg/mL, clear, colorless to light yellow
• storage temp.: −20°C

Descripción

General description

Gangliosides are major constituents of neuronal cell membranes and endoplasmic reticulum. They contain a sialated polysaccharide chain linked to ceramide through a β-glycosidic linkage. For classification of gangliosides see Svennerholm, L., et al. (eds.), Structure and Function of Gangliosides, New York, Plenum, 1980.

Application

Monosialoganglioside GM3 from canine blood has been used as a reference standard in high-performance thin-layer chromatography (HPTLC) to analyze ganglioside signatures of prostate cancer (CaP) cells. It has also been used to determine ganglioside-binding profiles of methylotrophic yeast (Pichia pastoris) secreted cholera toxin B subunit (PpCTB) by enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Monosialoganglioside GM3 has a potential role in cancer development and progression. Increased expression of GM3 has been observed in various types of cancer. Hence, it is used as a tumor-associated carbohydrate antigen during cancer vaccine development.

Monosialoganglioside-GM₃ is the most common ganglioside and the major ganglioside in non-neuronal cell membranes. It inhibits epidermal cell growth and is involved in the biosynthesis and metabolism of all higher gangliosides.

Analysis Note

The product is soluble at 20 mg/mL in a 1:1 mixture of chloroform and ethanol with 5% water. The resulting solution is colorless

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)