FD40S
Isotiocianato de fluoresceína-dextrano
average mol wt 40,000
Sinónimos:
FITC–Dextrano
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About This Item
Productos recomendados
origen biológico
synthetic (organic)
Nivel de calidad
conjugado
FITC conjugate
Formulario
powder
mol peso
average mol wt 40,000
Extensión del etiquetado
0.003-0.020 mol FITC per mol glucose
color
yellow to dark orange
solubilidad
H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
temp. de almacenamiento
2-8°C
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Descripción general
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9.
Aplicación
Dextrano marcado con isotiocianato de fluoresceína para su posible uso en estudios de perfusión en animales.
Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:
- Fluorescein-dextran microscopy.
- To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.
- To quantify endocytic activity by flow cytometry.
- To label α-lactalbumin and ribonuclease.
Ligadura / enlace
Similar to FD-40, but produced by Sigma.
Nota de preparación
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Otras notas
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Contenido relacionado
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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