D1946

Diaminofluorescein-FM diacetate

≥98% (HPLC)

• Sinónimos: 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy), DAF-FM DA
• Fórmula empírica (notación de Hill): C₂₅H₁₈F₂N₂O₇
• Número de CAS: 254109-22-3
• Peso molecular: 496.42
• Número MDL: MFCD16872050
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 329798717
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥98% (HPLC)
• Formulario: film
• condiciones de almacenamiento: desiccated
• color: colorless to yellow
• fluorescencia: λ_ex 500 nm; λ_em 515 nm in DMSO
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• cadena SMILES: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Clave InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Descripción

Aplicación

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Acciones bioquímicas o fisiológicas

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Características y beneficios

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Precaución

Hygroscopic

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable