The molecular weight of OptiPrep is 1552.2 amu, and its density is 1.320 g/ml. The prepared solution contains 60% OptiPrep. Based on these values, the calculated molarity is 0.706 Molar.
D1556
Medio para gradiente de densidad OptiPrep™
OptiPrep™ Density Gradient Medium
used for cell and subcellular organelle isolation
Sinónimos:
Iodixanol solution
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Quality Level
sterility
sterile
form
liquid
density
1.319-1.321 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
SMILES string
CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I
InChI
1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)
InChI key
NBQNWMBBSKPBAY-UHFFFAOYSA-N
General description
El medio de gradiente de densidad OptiPrep™, también conocido como yodixanol, es un medio de gradiente de densidad yodado no iónico de baja osmolaridad. Es una disolución estéril y comprobada para endotoxinas que contiene yodixanol al 60 % (p/v) en agua y está diseñada para el aislamiento in vitro de partículas biológicas. Se puede utilizar para el fraccionamiento y la separación de orgánulos celulares y subcelulares.[1]
Application
OptiPrep™ se ha utilizado para aislar moléculas, células y orgánulos subcelulares, como ADN, citocinas, lisosomas, retículo endoplásmico, peroxisomas, linfocitos T y linfocitos citolíticos naturales.[1] También se ha utilizado para las siguientes aplicaciones:
- investigar la eficiencia de la encapsulación unicelular.[1]
- el aislamiento basado en gradiente de las células cancerosas senescentes.[2]
- el aislamiento de viriones no encapsulados de los lavados broncoalveolares.[3]
- el aislamiento de lipoproteínas y macromoléculas
- purificación de viriones
- purificación de células neuronales
Features and Benefits
OptiPrep™ tiene numerosas propiedades biocompatibles que lo hacen ideal para su uso.
Other Notes
Disolución al 60 % (p/v) de iodixanol en agua (estéril).
Legal Information
OptiPrep is a trademark of Serumwerk Bernburg AG
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
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What is the molarity of OptiPrep (catalog number D1556)?
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What is the method used to determine the density mentioned on the Certificate of Analysis (CoA) for D1556 OptiPrep™ Density Gradient Medium?
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Based on the provided details, utilizing a densitometer or pycnometer is recommended.
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What is the density of OptiPrep: 60% v/v, w/w, or w/v?
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The density of OptiPrep is expressed as weight/volume (w/v). The stock concentration from the bottle is 60%.
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What is the osmolarity of OptiPrep?
1 answer-
According to the supplier information, the osmolarity of OptiPrep is stated to be 170 mOsm with a deviation of +/- 15 mOsm.
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What is the viscosity of the solution?
1 answer-
This product is not tested for viscosity. This item is a 60% solution of Iodixanol in water. However, Iodixanol properties reported by drugfuture.com state that a 50% solution has a viscosity of 8.6 mPa at 37°C.
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Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?
1 answer-
Percoll and Histopaque must be removed prior to use through washing. If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells. In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.
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How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?
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After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.
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What is the appearance of Product D1556, OptiPrep?
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The solution may be clear to slightly yellow.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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