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CS0720

Sigma-Aldrich

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Sinónimos:

Citrate Synthase Activity Test Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.28
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

300

uso

 kit sufficient for 100 reactions (using a 1 ml cuvette)
 kit sufficient for 480 reactions (using 96 multiwell plates)

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CS(1431)

Descripción general

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

Acciones bioquímicas o fisiológicas

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

Nota de análisis

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

Solo componentes del kit

Referencia del producto
Descripción
  • Assay buffer for citrate synthase 5x 25 mL
  • Bicine buffer 10 mL
  • CelLytic™ M Cell Lysis Reagent 10 mL
  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg
  • Acetyl coenzyme A 25 mg
  • Oxaloacetic acid 1 g
  • Citrate synthase (positive control) .1 mL

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000268900
0000397000
0000268900
0000397000
0000189694

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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What are the contents of the Assay Buffer (5x) for the CS0720 citrate synthase kit?

    1 answer

    1. The kit component B6935, the 5X assay buffer, comprises 0.5 M Tris HCl at pH 8.0.

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