CLLS1014
A549 Cells HIF1A -/-/-
human male lung (Source Disease: Lung carcinoma)
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About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
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Nombre del producto
A549 Cells HIF1A -/-/-,
origen biológico
human male lung (Source Disease: Lung Carcinoma)
Nivel de calidad
Nº de acceso OMIM
temp. de almacenamiento
−196°C
Información sobre el gen
human ... HIF1a(3091)
Descripción general
A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-185
Aplicación
Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Características y beneficios
These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
Calidad
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Solo componentes del kit
Referencia del producto
Descripción
- A549 Cells HIF1A -/-/- 1 vial
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Michal W Luczak et al.
Cell cycle (Georgetown, Tex.), 20(18), 1812-1827 (2021-08-13)
The hypoxia-induced transcription factor HIF1 inhibits cell growth in normoxia through poorly understood mechanisms. A constitutive upregulation of hypoxia response is associated with increased malignancy, indicating a loss of antiproliferative effects of HIF1 in cancer cells. To understand these differences
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