M15

95102517, mouse kidney, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: M15, 95102517
• biological source: mouse kidney
• growth mode: Adherent
• karyotype: Hyperdiploid
• morphology: Epithelial
• products: WT1 (expressed gene)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse mesonephric epithelium, polyoma virus large T transformed

Cell Line Description

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Application

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Other Notes

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