AT-3.1

94101451, rat skin, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: AT-3.1, 94101451
• origen biológico: rat skin
• modo de crecimiento: Adherent
• cariotipo: Hyperaneuploidy
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat dorsal prostatic adenocarcinoma

Descripción línea celular

AT-3.1 is a subclone of the AT-3 cell line being one of a series of related variants originating from a single spontaneous adenocarcinoma in the dorsal lobe of the prostate of an aged male inbred Copenhagen rat. AT-3.1 was derived from an anaplastic tumour with a high rate of metastases and is androgen-independent.

Aplicación

Carcinogenesis studies, cytogenetic analysis.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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