M3 Clone M-3

93120827, mouse skin, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: M3 Clone M-3, 93120827
• origen biológico: mouse skin
• modo de crecimiento: Adherent
• cariotipo: Modal no. 83; hypertetraploid
• morfología: Epithelial
• productos: Melanin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse Cloudman S 91 melanoma

Descripción línea celular

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Aplicación

Tumourigenicity studies

Medio de cultivo

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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