CTLL-2

93042610, mouse blood, Lymphoblast

• Sinónimos: CTLL-2 cell line
• UNSPSC Code: 41106514

Propiedades

• product name: CTLL-2, 93042610
• biological source: mouse blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C57b1/6 T cell

Cell Line Description

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

Application

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml.

Other Notes

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