ND-C

92090913, mouse nerve, Neuronal

• UNSPSC Code: 41106514

Propiedades

• product name: ND-C, 92090913
• biological source: mouse nerve
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.

Application

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm²; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO₂; 37°C.

Other Notes

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