ND15

92090907

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Rat Hybridoma nerve
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

The hybrid cell line ND15 was derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)- derived azaguanine-resistant line. HAT-sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3. When differentiated in serum free media an increased level of Thy-2 can be detected.

Application

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

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