C7902

Cloruro de calcio dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

• Fórmula empírica (notación de Hill): CaCl₂ · 2H₂O
• Número de CAS: 10035-04-8
• Peso molecular: 147.01
• Número CE: 233-140-8
• Número MDL: MFCD00149613
• Código UNSPSC: 12352302
• ID de la sustancia en PubChem: 24892994
• NACRES: NA.71

Propiedades

• grado: reagent
• Línea del producto: BioReagent
• Ensayo: ≥99.0%
• Formulario: powder
• técnicas: cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• mp: 176 °C (dec.) (lit.)
• solubilidad: H₂O: 294 mg/mL, clear, colorless
• cadena SMILES: O.O.Cl[Ca]Cl
• InChI: 1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
• Clave InChI: LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Descripción

Descripción general

Calcium chloride (CaCl₂) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl₂ is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl₂-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl₂ is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl₂ along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl₂ on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Aplicación

Calcium chloride dihydrate has been used:

• in the formulation of media
• in sperm collection and preparation
• in cell culture and protoplast preparation

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves