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Key Documents

C-14064

Sigma-Aldrich

Human Tracheal Epithelial Cells (HTEpC)

1 million cells per pellet

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human trachea

packaging

pkg of 1,000,000 cells

morphology

(epithelial)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Cell Line Origin

Trachea

Application

Primary Human Tracheal Epithelial Cells (HTEpC) are isolated from the surface epithelium of human trachea and stain positive for cytokeratin.The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HTEpC are useful for investigating the function and pathology of the respiratory system.

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

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