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B2787

Sigma-Aldrich

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

Sinónimos:

protein markers, protein molecular weight markers, protein standards

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About This Item

Código UNSPSC:
12352200
eCl@ss:
34058011
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

lyophilized powder

Nivel de calidad

200

uso

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

mol peso

6,500-180,000 Da

envase

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

temp. de almacenamiento

−20°C

Aplicación

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.

Componentes

This product contains:
  • α2-macroglobulin (equine serum)
  • β-galactosidase (E. coli)
  • phosphorylase b (rabbit muscle)
  • catalase (bovine liver)
  • alcohol dehydrogenase (yeast)
  • carbonic anhydrase (bovine erythrocyte)
  • trypsin inhibitor (soy bean)
  • lysozyme (chicken egg)
  • aprotinin (bovine lung)
Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Otras notas

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitución

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLBW9707
SLBT3042
SLBF2970
091M6214
080M6115

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D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

Questions

1–2 of 2 Questions  

  1. The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C.  What information can you provide?

    1 answer

    1. Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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