A9253

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

• Sinónimos: L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)
• Número de CAS: 9000-89-9
• Comisión internacional de enzimas: 1.4.3.2 (BRENDA, IUBMB)
• EC Number: 232-564-0
• MDL number: MFCD00131454
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: Crotalus adamanteus
• Quality Level: 200
• type: Type I (dried venom); Type I
• form: powder
• mol wt: ~130 kDa
• solubility: H₂O: soluble 1.0 mg/mL, clear(lit.)
• storage temp.: −20°C

Descripción

General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa.[1] Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules.[2] LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5.[3] It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney.[3] In the N-terminal region, it has a βαβ domain with glutamic acid residues.[3] LAAO imparts yellow color to venom.[4]

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.[5]

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays.[6] The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.[7]

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg²⁺ for its activation.[3] The N-terminal region is essential for FAD binding.[3] The H₂O₂ generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis.[4] LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).[8]

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Información de seguridad

• pictograms: GHS06
• signalword: Danger
• hcodes: H300 + H310 + H330
• pcodes: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable