The production process for A7641 does not involve the use of tris. All pH adjustments and chromatography steps are carried out using Acetic acid or Ammonium hydroxide. The material is essentially free of salts before undergoing lyophilization, although there is a possibility of trace amounts of NaCl due to chromatography. The NaCl concentration would be less than 0.01%.
A7641
Albúmina from chicken egg white
lyophilized powder
Sinónimos:
Albúmina from hen egg white, Ovalbúmina
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About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25
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Productos recomendados
biological source
chicken egg white
Quality Level
type
Grade VII
assay
≥95% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
44287 Da by calculation
purified by
chromatography
technique(s)
bioconjugation: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... OVAL(396058)
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General description
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa. [1]
Ovalbumin, an egg white protein is produced in the oviduct of hen. It has 3 sites of post synthetic modification and an N-terminal acetyl group.[2] This product is free of S-ovalbumin, a heat stable form of egg albumin formed when eggs are stored.
Application
Glucoproteína fosforilada que es útil como marcador (~45 kDa) para la electroforesis de proteínas. Se ha utilizado para producir un modelo animal del asma.
Albumin from chicken egg white has been used:
- as a component in reaction buffer for in vitro enzymatic assay[3]
- to simulate dangerous protein toxins[4]
- as a component in marker solution[5]
The product is useful as a marker (∼45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma.
Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.[6]
It was also used in a study testing intestinal macromolecule absorption in fetal pig after infusion of colostrum in utero.[7]
Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.[6]
It was also used in a study testing intestinal macromolecule absorption in fetal pig after infusion of colostrum in utero.[7]
Biochem/physiol Actions
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C.[8] Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.
Ovalbumin is preferred as a nutrient supplement. It can serve as a drug carrier. It possess tumor necrosis releasing factors, that can play a great role in tumor suppression.[2]
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1A
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The ability of the fetal pig intestine to absorb large proteins was investigated in utero. Six pregnant sows were anesthetized (Na pentobarbital) at 99-102 d of gestation (term = 115 +/- 2 d), and a catheter was inserted into the
Artículos
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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Is there any information available regarding whether chicken egg white albumin product A7641 was prepared in a buffer solution containing tris prior to lyophilization, or if it was prepared from a pure form of chicken egg white albumin without any buffer?
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