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Merck

A7095

Sigma-Aldrich

Aminoglucosidasa from Aspergillus niger

≥260 U/mL, aqueous solution

Sinónimos:

1,4-α-D-Glucano glucohidrolasa, Exo-1,4-α-glucosidasa, Glucoamilasa

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source

Aspergillus niger

Quality Level

form

aqueous solution

specific activity

≥260 U/mL

density

~1.2 g/mL at 25 °C

storage temp.

2-8°C

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General description

Stabilized with glucose.
Amyloglucosidase is a disaccharidase−type alpha-glucosidase,[1] produced by several species of Aspergillus genus.[2] Immobilization of amyloglucosidase is known to increased its stability.[3]

Application

Amyloglucosidase from Aspergillus niger has been used in in vitro digestions.[4] It has also been used in the isolation of insoluble and soluble dietary fibre from quinoa and amaranth.[5]

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[6] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.[7][8]

Legal Information

A product of Novozymes Corp.
AMG is a trademark of Novozymes Corp.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Fandila Carlos-Amaya et al.
Journal of agricultural and food chemistry, 59(4), 1376-1382 (2011-01-11)
Banana starch was chemically modified using single (esterification or cross-linking) and dual modification (esterification-cross-linking and cross-linking-esterification), with the objective to increase the slowly digestible starch (SDS) and resistant starch (RS) concentrations. Physicochemical properties and in vitro digestibility were analyzed. The
Quinoa (Chenopodium quinoa W.) and amaranth (Amaranthus caudatus L.) provide dietary fibres high in pectic substances and xyloglucans
Lamothe Lisa M, et al.
Food Chemistry, 167(4), 490-496 (2015)
Recent Advances in Basic and Applied Aspects of Industrial Catalysis, 891-891 (1998)
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries, 12(5), 183-183 (2000)
Effect of two barley beta-glucan concentrates on in vitro glycaemic impact and cooking quality of spaghetti
Chillo, S and Ranawana, DV and Henry, CJK
Food Sci. Technol., 44(4), 940-948 (2011)

Questions

1–3 of 3 Questions  
  1. What is the optimum temperature of this enzyme?

    1 answer
    1. The optimal temperature is 70 - 75 °C

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  2. Can I dilute the amyloglucosidase enzyme (A7095) in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer
    1. Unfortunately, the formulation of this solution is considered proprietary. There is no information regarding further dilutions, however the optimum pH is 4.5. Any aqueous diluent should like to stay in this approximate range. See the table below to review the pH on enzyme activity.

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  3. Could you please provide the source of the A7095 and the molecular weight of the enzyme? and is this a pure enzyme?

    1 answer
    1. The source of this product can be found on the lot specific Certificate of Origin. This product is from species ASPERGILLUS NIGER. Please access a Certificate of Origin in the DOCUMENTATION section under 'Certificate of Origin':
      https://www.sigmaaldrich.com/product/sigma/a7095#product-documentation

      Amyloglucosidase from A. niger exists as two isoforms with molecular weights of 69,810 kDa and 89,130 kDa, as determined by SDS-PAGE. See: Starch, 34, 346 (1982). The units/mL is determined per batch as reported in Certificate of Analysis. The purity is not part of product specification and is not determined.

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