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Merck

30326

Sigma-Aldrich

EMPIGEN® BB detergent

~30% active substance

Sinónimos:

N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine

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About This Item

Fórmula lineal:
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
Número de CAS:
Beilstein:
3670807
Número CE:
Número MDL:
Código UNSPSC:
12161900
ID de la sustancia en PubChem:
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

descripción

zwitterionic

Nivel de calidad

Formulario

liquid

composición

solid content, 35.0-45.0 % (w/v)

concentración

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

densidad

1.030-1.060 g/mL at 20 °C

Absorción UV

λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037

cadena SMILES

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

Clave InChI

DVEKCXOJTLDBFE-UHFFFAOYSA-N

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Aplicación

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity[1]. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease[2]. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses[3].

Información legal

EMPIGEN is a registered trademark of Huntsman Corporation

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

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James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
Wenjin Guo et al.
Protein expression and purification, 90(1), 34-39 (2013-05-15)
Vaccinia virus (VV) has been used to express a variety of heterologous proteins, including HIV envelope (Env) glycoproteins. The Env protein is synthesized as a precursor molecule, gp160, which is cleaved into the surface antigen gp120 and the transmembrane protein
Islam Khan et al.
Microbes and infection, 7(5-6), 853-859 (2005-05-10)
Campylobacter jejuni is a major food-borne pathogen and a leading cause of diarrhoea. A cytotoxin is most likely involved in the pathogenesis of inflammatory diarrhoea due to C. jejuni. A 45-kDa outer membrane protein encoded by the porA gene was
R Jennings et al.
Journal of medical virology, 31(2), 98-108 (1990-06-01)
HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. 이 용액은 증류수에 30% 희석되어 있는 건가요? 아니라면 희석한 용액(active substance)이 무엇인지 궁금합니다.

    1 answer
    1. This product is a 30% solution in water. The active substances are chemically identified as C12-14-Alkyldimethyl-betaines. Please see the link below to review the product Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/30326

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