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Merck

N4638

Sigma-Aldrich

Neutral Red

BioReagent, Powder

Sinónimos:

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Fórmula empírica (notación de Hill):
C15H17ClN4
Número de CAS:
Peso molecular:
288.78
Colour Index Number:
50040
Beilstein/REAXYS Number:
3918740
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Neutral Red, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

form

powder

composition

Dye content, ≥90%

technique(s)

cell culture | mammalian: suitable
titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:
  • as a nuclear stain
  • as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
  • fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
  • detection of viable yeast cells in dandruff samples and of mussel larvae
  • Neutral red has also been used as a stain in flow cytometry.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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This study introduces a novel and simple method to covalently graft neutral red (NR) onto carbon surfaces based on spontaneous reduction of in situ generated NR diazonium salts. Immobilization of neutral red on carbon surface was achieved by immersing carbon

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