B4501

Biotina

≥99% (HPLC), lyophilized powder

• Sinónimos: D-Biotina, Bios II, Coenzima R, Vitamina H, Vitaminas B7
• Fórmula empírica (notación de Hill): C₁₀H₁₆N₂O₃S
• Número de CAS: 58-85-5
• Peso molecular: 244.31
• Beilstein: 86838
• Número CE: 200-399-3
• Número MDL: MFCD00005541
• Código UNSPSC: 12352201
• eCl@ss: 34058011
• ID de la sustancia en PubChem: 24891778
• NACRES: NA.21

Propiedades

• origen biológico: synthetic (organic)
• Nivel de calidad: 300
• Ensayo: ≥99% (HPLC)
• Formulario: lyophilized powder
• técnicas: HPLC: suitable
• color: white to off-white
• mp: 231-233 °C (lit.)
• solubilidad: ammonium hydroxide: 50 mg/mL (2 M)
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
• Clave InChI: YBJHBAHKTGYVGT-ZKWXMUAHSA-N

Descripción

Descripción general

Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources.[1] Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.[2]

Aplicación

Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:

• for culturing of oligodendrocytes.[3][4]
• as a vitamin supplement for the growth of Bacillus species.[5]
• for blocking endogenous biotin during immunohistology procedures.[6][7]

Acciones bioquímicas o fisiológicas

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC).[1] It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.[1]

Otras notas

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Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)