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B0126

Sigma-Aldrich

Bromophenol Blue

Powder

Sinónimos:

3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form

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About This Item

Fórmula empírica (notación de Hill):
C19H10Br4O5S
Número de CAS:
115-39-9
Peso molecular:
669.96
Beilstein:
61698
Número CE:
204-086-2
Número MDL:
MFCD00005875
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24891504
NACRES:
NA.47

Nombre del producto

Bromophenol Blue, titration: suitable

Agency

suitable for SM 2310
suitable for SM 2320

Nivel de calidad

200

Formulario

powder

técnicas

titration: suitable

color

faint beige to very dark beige
faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

solubilidad

methanol: 10 mg/mL

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

Clave InChI

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Descripción general

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicación

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000422767
0000422767
SHBR8142
0000360699
0000280442

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