67309

Kovac′s reagent for indoles

suitable for microbiology

• Sinónimos: 4-(Dimethylamino)benzaldehyde solution
• Fórmula empírica (notación de Hill): C₉H₁₁NO
• Número de CAS: 100-10-7
• Peso molecular: 149.19
• Beilstein: 4132845
• Número MDL: MFCD00003381
• Código UNSPSC: 41171621
• ID de la sustancia en PubChem: 329760541
• NACRES: NA.85

Propiedades

• Agency: according to ISO 16654:2001
• Nivel de calidad: 200
• Línea del producto: BioChemika
• caducidad: limited shelf life, expiry date on the label
• composición: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• técnicas: microbe id | specific enzyme detection: suitable
• aplicaciones: agriculture; clinical testing; environmental; food and beverages; microbiology
• temp. de almacenamiento: 2-8°C
• idoneidad: Escherichia coli; coliforms
• cadena SMILES: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• Clave InChI: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Descripción

Descripción general

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Aplicación

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

Información de seguridad

• Pictogramas: GHS02,GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H226,H290,H314,H317,H332,H335
• Consejos de prudencia: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 3 - Flammable liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): 109.4 °F
• Punto de inflamabilidad (°C): 43 °C
• Equipo de protección personal: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter