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Merck

51558

Sigma-Aldrich

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

Sinónimos:

HEPES Saline Buffer Solution

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About This Item

Número MDL:
Código UNSPSC:
12161700
ID de la sustancia en PubChem:
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

Línea del producto

BioUltra

Formulario

liquid

calidad

2× concentrate

composición

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentración

2 ×

impurezas

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

índice de refracción

n20/D 1.339

pH

7.2 (25 °C)

trazas de catión

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorción UV

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

cadena SMILES

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Clave InChI

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES buffered saline is a tissue culture buffer,[1] which is used to maintain the pH of cell culture.[2] It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.[3]

Aplicación

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA[4][5]
  • as a reagent to prepare transfection master mix for DNA construct vectors[6]
  • to develop hydrogel precursor for cell leakage assay[7]

Componentes

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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Certificados de análisis (COA)

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Slide 1 of 4

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A new approach to neural cell culture for long-term studies
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Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
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Kingston RE, Chen CA, Okayama H.
Current Protocols in Immunology, 10, 10-10 (2001)
Labeling embryonic mouse central nervous system cells by in utero electroporation
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Development, Growth & Differentiation, 50(6), 507-511 (2008)
Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Cell Biology, 20, 20-20 (2003)
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B11 (1989)

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. whether the HEPES is dissolved in PBS? Additionally, how about the concentration of the HEPES in PBS?

    1 answer
    1. Per the components section on the Product Detail page, the composition of the product is as follows: concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose.

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