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UFC9100

Millipore

Filtro de ultracentrífuga Amicon®, MWCO 100 kDa

sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

Sinónimos:

Concentrador para centrífuga, MWCO de 100 kDa, volumen de muestra 15 ml

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
En este momento no podemos mostrarle ni los precios ni la disponibilidad

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

15 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm2

volume

15 mL

pore size

100 kDa MWCO

shipped in

ambient

Categorías relacionadas

General description

Las unidades de centrífuga Amicon® Ultra de 15 ml con filtros Ultracel® de corte de peso molecular de 100 000 Dalton proporcionan una ultrafiltración rápida y una retención fiable de las macromoléculas. Por ejemplo, la IgG (156 kDa) a una concentración inicial de 1 mg/ml es retenida al >90 %.

El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 80 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra.

El concentrado se recoge del depósito de muestras de la unidad de filtración utilizando un pipeteador, mientras que el ultrafiltrado se recoge en el tubo de centrífuga suministrado. La unidad puede centrifugarse en un rotor oscilante o en un rotor de ángulo fijo. Con su diseño de membrana vertical y bloqueo, los filtros Amicon® Ultra de 15 ml ofrecen la mayor recuperación con tiempos de centrifugado cortos.

Application

  • Ultrafiltración con membrana Ultracel® de corte de peso molecular de 100 000 dalton.
  • Concentración de muestras biológicas que contienen antígenos, anticuerpos, enzimas, ácidos nucleicos o microorganismos.
  • Purificación de los componentes macromoleculares encontrados en extractos de cultivo tisular o lisados celulares y eliminación de proteínas antes de la HPLS.
  • Concentración de las proteínas diluidas o prepurificadas de los eluyentes de la columna.
  • Desalación, cambio de disoluciones tampón y diálisis de proteínas.

Features and Benefits

  • La membrana de celulosa regenerada Ultracel® con un NMWCO de 100 kDa proporciona una recuperación de retenido superior al 90 %.
  • La membrana vertical reduce la polarización de la concentración para tiempos de centrifugado ultrarrápidos (de hasta 10 – 15 minutos).
  • La membrana termosellada minimiza los extraíbles posteriores.
  • Integridad probada al 100 % para un rendimiento fiable.
  • Cómoda supervisión de la muestra con carcasa traslúcida y gradaciones de volumen.
  • El acceso directo a la muestra con pipeteador elimina la etapa de procesamiento para recuperar el concentrado.
  • Factores de concentración elevada de 80 – 100 X.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Contenido relacionado

Amicon® Ultra Centrifugal Filters

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

Questions

1–10 of 12 Questions  

  1. Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?

    1 answer

    1. These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration

      Helpful?

  2. Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks

    1 answer

    1. Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  3. What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?

    1 answer

    1. This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
      https://www.sigmaaldrich.com/product/mm/ufc5100

      Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
      https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765

      Helpful?

  4. Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?

    1 answer

    1. These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  5. I just noticed that Amicon ultra - 15 centrifugal filters are non-sterile. Has this been always the case? REF: UFC910024, LOT:0000193199

    1 answer

    1. The Amicon Ultra devices have always been non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

      Helpful?

  6. What is the maximum volume that can be loaded into the Amicon 15 device when using both fixed angle and swinging bucket rotor centrifuges?

    1 answer

    1. When using the fixed angle centrifuges with the Amicon 15 device, the maximum volume that can be loaded is 12 mL. It is important to place the device at a 35-degree angle. On the other hand, when using swinging bucket centrifuges, the maximum volume that can be loaded into the Amicon 15 device is 15 mL.

      Helpful?

  7. Hi, how should we place the amicon ultra-15 tube in the fixed-angle rotor, (could you please explain the sentence "When using a fixed-angle rotor, orient the device with the membrane panel facing up" , sorry I'm not a native English speaker

    1 answer

    1. When using a fixed-angle rotor, orient the device with the membrane panel facing up as demonstrated in the image below.

      For a more detailed view, kindly review the attached video link: https://youtu.be/GTH7kQMUjFg?feature=shared&t=145. This video also discusses the relative size of each Amicon® Ultra Centrifugal Filter and what kind of rotors they will fit into.

      Helpful?

  8. Hii, I wold like use these tubes for ultrafiltration of FBS to deplete it from EVs. I found they were used in one publication for such an experiment. How can they be sterilized so they can be used in cell culture hoods?

    1 answer

    1. Amicon Ultra devices cannot be sterilized by UV or autoclaving. These products are intended as a 1 time use device, however it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from the device as previously described prior to use.

      Helpful?

  9. Hi, Can you please confirm the centrifugation speeds ( in rcf ) for the Amicon-15 100kDa and the 10kDa filters ? I was told that the max speed for the 100kDa is 3000g and for the 10kDa is 4000g, respectively. Is that the case and if so, why exactly ?

    1 answer

    1. Spin times range from 10 - 40 minutes and will depend on the sample types as well as the rotor (fixed-angle or swinging bucket). See the image below for spin times and speeds for each rotor type.
      Please see the link below to review the product FAQs for additional usage information:
      https://www.emdmillipore.com/US/en/product/Amicon-Ultra-15-Centrifugal-Filter-Unit,MM_NF-UFC910008#documentation

      To request the product user guide, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further.

      Helpful?

  10. Please could you provide the manual for these kind of filters: Amicon Ultra 15 ml centrifugal filters

    1 answer

    1. Kindly review the user guide for these products at the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/360/134/pr05035-rev1018.pdf

      Helpful?

1–10 of 12 Questions  

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