SCR713M
TagGFP2 Simplicon RNA
to determine optimal transfection conditions to express the self-replicating RNA in cell lines
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.51
Productos recomendados
Quality Level
technique(s)
nucleic acid detection: suitable (RNA)
General description
Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1. The Simplicon RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been completely removed and replaced with the transgene(s) of interest. Because Simplicon is a synthetic RNA, there is no risk of the transgene(s) integrating into the transfected cell’s genome. Moreover, the self-replicating nature of the Simplicon means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc1.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
Application
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells.
Components
1. TagGFP2 RNA: (Part No. CS222751) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
Quality
• Discrete RNA band on RNA gel of appropriate size.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico