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Key Documents

MABF2235

Sigma-Aldrich

Anti-Enterovirus 71 3D Antibody, clone 4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

clone

4
monoclonal

purified by

using protein G

species reactivity

mouse, virus

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

Recombinant 3D polymerase from Enterovirus 71.

Specificity

Clone 4 is a mouse monoclonal antibody that specifically detects Enterovirus 71 3D polymerase in EV71 infected cells.

Application

Immunofluorescence Analysis: A representative lot detected Enterovirus 71 3D in Immunofluorescnece applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Enterovirus 71 3D in Immunoprecipitation applications (Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).

Western Blotting Analysis: A representative lot detected Enterovirus 71 3D in Western Blotting applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, H.I., et. al. (2018). PLoS One. 13(1): e0191617).

Quality

Evaluated by Western Blotting in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Enterovirus 71 3D in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.

Physical properties

~52 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Virus (Enterovirus)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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