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Key Documents

AG962

Sigma-Aldrich

β Amyloid 1-40, aβ, ultra pure, HFIP, recombinant human

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

assay

>97%

form

powder

manufacturer/tradename

Chemicon®

UniProt accession no.

Gene Information

human ... APP(351)

General description

Beta Amyloid (1-40), ultra pure, HFIP.

MOL. WT.: 4329.86 Da by Mass Spec Analysis (theoretical 4329.86)

SEQUENCE: Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val

PEPTIDE CONTENT: 1 mg per vial; no salt as HFIP desalts the sample.

COUNTER ION: HFIP

Application

Different researchers claim enhanced activity due to different salts.



The TFA salt is what has been the industry standard.



The HFIP makes the beta-amyloid homogenously monomeric.



The NaOH apparently make it monomeric too and is also water soluble, which the TFA salt and HFIP are not.



The HCl salt is supposed to be more biological, as TFA is not found in the body.



By offering the different salts, it lets the researchers duplicate the different assays that have been developed by other researchers (see below).



TFA: Shu-chuan Jao, S-C, et. al. 1997, Amyloid; Int J Exp. Clinical Invest. 4, 231

HFIP: Stine, W.B. et. al. 2003, J. Biol. Chem, 278, 11612

NaOH: Fezoui, Y., et. al. 2000, Amyloid, 7, 166

HCL: Kanek, I and Tutumi, S., 1997, J. Neurochem, 68, 438
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Physical form

White lyophilized powder. Resuspend in 1% NH4OH at a concentration of 1 mg/mL. Sonicate for 30 seconds to 1 minute after it has gone into solution. To bring into your solution: After resuspension, add 5x or 10x buffer stock (PBS, TBS) and water to bring to 1x buffer.

Storage and Stability

Maintain lyophilized material at -20ºC for up to 12 months after date of receipt. After reconstitution maintain at -20ºC to -70ºC for up to 2 weeks in undiluted aliquots. Avoid freeze/thaw cycles to avoid aggregation.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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