ABS2210

Anti-ANO6/TMEM16F

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody; serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• mol peso: calculated mol wt 55.73 and 106.26 kDa; observed mol wt ~58 and 110 kDa
• purificado por: (Unpurified)
• reactividad de especies: mouse
• envase: antibody small pack of 100 μL
• técnicas: western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_780553
• Nº de acceso UniProt: Q6P9J9
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... ano6> Ano6(105722)

Descripción

Descripción general

Anoctamin-6 (UniProt: Q6P9J9; also known as Small-conductance calcium-activated nonselective cation channel, SCAN channel, Transmembrane protein 16F) is encoded by the Ano6 (also known as Tmem16f) gene (Gene ID: 105722) in murine species. ANO6/TMEM16F is a multi-pass membrane glycoprotein with six cytoplasmic domains, ten transmembrane domains, and four extracellular domains. It is predominant expressed in epithelial tissues and is also found in skeletal system where it is primarily expressed in osteoblasts. It serves as a small-conductance calcium-activated nonselective cation (SCAN) channel that acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system. In osteoblasts it is essential for the deposition of hydroxyapatite during bone mineralization. ANO6/TMEM16F displays calcium-dependent phospholipid scramblase activity whereby it scrambles phosphatidylserine, phosphatidylcholine, and galactosylceramide. It is shown to be a key component of the plasma membrane repair mechanism triggered by pore-forming agents, such as bacterial toxins. This is achieved by activation of ANO6/TMEM16F by an increase in intracellular calcium resulting from flux of extracellular calcium ion. ANO6/TMEM16F is also shown to generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat cells . (Ref.: Wu, N., et al. (2020). Cell Rep. 30(4); 1129-1140).

Especificidad

This rabbit polyclonal antibody detects Anoctamin-6 (TMEM16F) in murine species. It targets and epitope within the C-terminal region and detects both isoforms 1 and 2.

Inmunógeno

GST-tagged recombinant fragment corresponding to 65 amino acids from the C-terminal region of murine Anoctamin-6 (TMEM16F).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Mouse thymus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANO6/TMEM16F in Mouse thymus tissue lysate.

Tested Applicaitons

Western Blotting Analysis: A representative lot detected ANO6/TMEM16F in Western Blotting applications (Wu, N., et. al. (2020). Cell Rep. 30(4):1129-1140.e5).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ANO6/TMEM16F, Cat. No. ABS2210, is a rabbit polyclonal antibody that detects Anoctamin-6 and is tested for use in Western Blotting.

Forma física

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Almacenamiento y estabilidad

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable