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Merck
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Key Documents

17-500M

Millipore

Catch and Release v2.0 Reversible Immunoprecipitation System

Sinónimos:

Catch and release system, Immunoprecipitation kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NB.22

species reactivity (predicted by homology)

all

Quality Level

manufacturer/tradename

Catch And Release
Upstate®

technique(s)

activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

General description

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

Application

Catch and Release v2.0 should not be used with proteins containing a 6-His tag.

Packaging

50 immunoprecipitations

Components

Catch and Release Wash Buffer, 10X (Cat.# 20-210)

Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)

Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)

20-285

20-216

Catch and Release Capture Tubes

Quality

routinely evaluated by immunoprecipitation.

Storage and Stability

All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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