06-418
Anti-NFκB p65 Antibody, CT
Upstate®, from rabbit
Sinónimos:
Nuclear factor NF-kappa-B p65 subunit, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear
factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel r
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
mouse, rat, human
fabricante / nombre comercial
Upstate®
técnicas
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... NFKB1(4790)
Descripción general
The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
Especificidad
NFκB p65, Mr 65 kDa
Inmunógeno
Epitope: C-terminus
KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.
Aplicación
Anti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Calidad
Routinely evaluated by Western Blot on RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Descripción de destino
65 kDa
Ligadura / enlace
Replaces: 04-1008; 04-235
Forma física
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Nota de análisis
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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