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Merck
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Documentos clave

04-300

Sigma-Aldrich

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate®, from mouse

Sinónimos:

INSR

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

21g12, monoclonal

reactividad de especies

canine, human, mouse

fabricante / nombre comercial

Upstate®

técnicas

ELISA: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pTyr1322)

Información sobre el gen

human ... INSR(3643)

Especificidad

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Inmunógeno

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Aplicación

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Calidad

Routinely evaluated by immunoblot.

Descripción de destino

97 kDa

Forma física

Format: Purified
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Subsequent thiophilic adsorption and size exclusion chromatography

Almacenamiento y estabilidad

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Includes pervanadate treated HEK293 lysate as a positive control.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Francesca Fiory et al.
Molecular and cellular biology, 25(24), 10803-10814 (2005-11-30)
In L6 myoblasts, insulin receptors with deletion of the C-terminal 43 amino acids (IR(Delta43)) exhibited normal autophosphorylation and IRS-1/2 tyrosine phosphorylation. The L6 cells expressing IR(Delta43) (L6(IRDelta43)) also showed no insulin effect on glucose uptake and glycogen synthase, accompanied by
Qiu-yan Wang et al.
The FEBS journal, 274(2), 526-538 (2007-01-19)
Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of alpha1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In alpha1,3-FucT-VII-transfected cells, expression of insulin receptor (InR) alpha- and beta subunits and epidermal growth factor receptor
Template to improve glycemic control without reducing adiposity or dietary fat.
Krishnapuram, R; Dhurandhar, EJ; Dubuisson, O; Kirk-Ballard, H; Bajpeyi, S; Butte et al.
American Journal of Physiology. Endocrinology and Metabolism null
Frankie B Stentz et al.
Biochemical and biophysical research communications, 335(2), 491-495 (2005-08-09)
Upon activation by phytohemagglutine (PHA), T-lymphocytes (T-cells) express receptors for growth factors, insulin, IGF-1 and IL2 and become insulin sensitive. Diabetic ketoacidosis (DKA) is associated with in vivo emergence of these growth factor receptors without incubation with PHA. As DKA
Molecular cloning of a DNA sequence complementary to creatine kinase M mRNA from chickens.
Schweinfest, CW; Kwiatkowski, RW; Dottin, RP
Proceedings of the National Academy of Sciences of the USA null

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