Skip to Content
Merck
All Photos(1)

Documents

SRP0293

Sigma-Aldrich

Cathepsin V Active human

recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Synonym(s):

Cathepsin L2, Cathepsin U

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in FreeStyle™ 293-F cells

Assay

≥90% (SDS-PAGE)

form

aqueous solution

specific activity

≥436 pmol/min-μg

mol wt

37 kDa

NCBI accession no.

NM_001333.2

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSL2(1515)

General description

Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Application

Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service