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SML3797

Sigma-Aldrich

Biotin-HPDP

≥95% (HPLC)

Synonym(s):

(3aS,4S,6aR)-Hexahydro-2-oxo-N-[6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]-1H-thieno[3,4-d]imidazole-4-pentanamide, HPDP-Biotin, N-[6-(Biotinamido)hexyl]-3′-(2′-pyridyldithio)propionamide

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About This Item

Empirical Formula (Hill Notation):
C24H37N5O3S3
CAS Number:
129179-83-5
Molecular Weight:
539.78
MDL number:
MFCD00078541
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

powder

storage condition

protect from light

color

white to beige

solubility

DMSO: 2 mg/mL, clear (Warmed)

storage temp.

-10 to -25°C

Biochem/physiol Actions

Biotin-HPDP (HPDP-Biotin) is a membrane-permeable and reversible biotinylating reagent that is used to biotinylate target proteins via covalent disulfide bond (-S-S-) formation with free sulfhydryl (-SH) groups. The biotin-tagged proteins can then be subjected to affinity labeling or isolation/purification using avidin conjugates or beads. Unlike tranditonal biotinylation methods, Biotin-HPDP tag can be removed with reducing agents such as dithiothreitol (DTT) without the need of harsh reagents to dissociate the high affinity interaction between avidin and biotin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Localization and partial characterization of the oligomeric disulfide-linked molecular weight 95,000 protein (triadin) which binds the ryanodine and dihydropyridine receptors in skeletal muscle triadic vesicles
Biochemistry, 30(30), 7507-7513 (1991)
Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins
Free Radical Biology & Medicine, 44(7), 1362-1372 (2008)
Low-Background Acyl-Biotinyl Exchange Largely Eliminates the Coisolation of Non- S-Acylated Proteins and Enables Deep S-Acylproteomic Analysis
Analytical Chemistry, 91(15), 9858-9866 (2019)

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