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SAB4200235

Sigma-Aldrich

Anti-TGN46 antibody, Mouse monoclonal

clone TGN46-52, purified from hybridoma cell culture

Synonym(s):

Anti-Trans-Golgi network protein, 46-kD

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About This Item

MDL number:
MFCD09265378
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TGN46-52, monoclonal

form

buffered aqueous solution

mol wt

antigen 80-100 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TGN46(10618)

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General description

Monoclonal Anti-TGN46 (mouse IgG1 isotype) is derived from the hybridoma TGN46-52 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TGN46 conjugated to KLH. Trans-Golgi network protein 46-kDa (TGN46), It is a heavily glycosylated protein, containing a signal peptide, a lumenal domain, a membrane-spanning domain and a cytoplasmic domain. The membrane spanning region and the cytoplasmic tail contain the retention and retrieval signals for localization in the TGN.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-TGN46 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Trans-Golgi network protein, 46-kDa (TGN46) is involved in regulating membrane traffic to and from the TGN. It cycles constitutively between the TGN and the plasma membrane, returning via endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Haik Mkhikian et al.
eLife, 5 (2016-06-09)
Essential biological systems employ self-correcting mechanisms to maintain cellular homeostasis. Mammalian cell function is dynamically regulated by the interaction of cell surface galectins with branched N-glycans. Here we report that N-glycan branching deficiency triggers the Golgi to generate bioequivalent N-glycans
A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface
Wakana Y, et al.
The Embo Journal, 31(20), 3976-3990 (2012)
Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis
Mkhikian H, et al.
eLife, 5(20), e14814-e14814 (2016)
Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network
Liljedahl M, et al.
Cell, 104(3), 409-420 (2001)
Maorong Chen et al.
eLife, 9 (2020-09-15)
Wnt signaling through the Frizzled (FZD) family of serpentine receptors is essential for embryogenesis and homeostasis, and stringent control of the FZD protein level is critical for stem cell regulation. Through CRISPR/Cas9 genome-wide screening in human cells, we identified TMEM79/MATTRIN

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