M9695
Matrix Metalloproteinase-12, Catalytic Domain human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MMP-12, Macrophage Elastase
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
calculated mol wt 20.3 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MMP12(4321)
General description
Matrix metalloproteinase-12 (MMP-12) is also called as macrophage metalloelastase and is secreted by inflammatory macrophages. It is synthesized as a zymogen and then activated by cleaving the propeptide domain. The MMP-12 gene is localized on chromosome 11.
Biochem/physiol Actions
MMP-12 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.
Matrix metalloproteinase-12 (MMP-12) breaks down proteoglycans, collagen type IV and laminin. Its main substrate is elastin.
Unit Definition
One unit equals 100 pmol/min at 37 °C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt as substrate.
Physical form
Solution in 50 mM Tris, 5 mM calcium chloride, 500 mM sodium chloride, 20 μM zinc chloride, 0.5% Brij® L23, and 30% glycerol, pH 9.5.
Legal Information
Brij is a registered trademark of Croda International PLC
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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