H6666

Keratinocyte Growth Factor human

HumanKine^®, >95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(s): FGF-7, KGF
• CAS Number: 148348-15-6
• MDL number: MFCD00212245
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Keratinocyte Growth Factor human, KGF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 4-20 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 17-30 kDa (glycosylated)
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• UniProt accession no.: P21781
• storage temp.: −20°C
• Gene Information: human ... FGF7(2252)

Description

General description

Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family. It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs. The KGF gene is located on the human chromosome at 15q21.2.

Application

Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.

Biochem/physiol Actions

Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs. KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable