A4475

Anti-Annexin VII antibody, Mouse monoclonal

clone 203-217/6, purified from hybridoma cell culture

• Synonym(s): Synexin
• MDL number: MFCD03792412
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 203-217/6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 47-51 kDa
• species reactivity: mouse, canine, rat, chicken, bovine, human, rabbit
• technique(s): immunocytochemistry: suitable using methanol or 1% paraformaldehyde/0.1% glutaraldehyde; immunohistochemistry (frozen sections): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using whole cell extract of culture mouse connective tissue (A9) cells.
• isotype: IgG1
• UniProt accession no.: P20073
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ANXA7(310) ; mouse ... Anxa7(11750) ; rat ... Anxa7(155423)

Description

General description

Annexin VII (synexin) is a membrane binding protein that localizes in the nucleus and cytosol. In humans and mice, Annexin VII is encoded by a single gene that has alternatively spliced cassette exons. As a result, two isoforms of molecular mass of 47kDa and 51kDa are expressed. These isoforms differ in their N-terminal region and also have distinct tissue-specific expression patterns.

Anti-Annexin VII antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 203-217/6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant mouse Annexin VII. The human annexin VII (ANXA7) is located on chromosome 10q21.

Specificity

Mouse monoclonal anti-annexin VII antibody reacts with human, bovine, rabbit, dog, rat, mouse, and chicken Annexin VII.

Immunogen

recombinant mouse annexin VII.

Application

Anti-Annexin VII antibody, Mouse monoclonal has been used in:

• western blotting
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

Antibodies reacting specifically with Annexin VII serve as an essential tool for the detection of this molecule in various cells. The human annexin VII (ANXA7) possesses a tumor suppressor role in prostate cancer. Hence it can be used an important biomarker along with syndecan-1 to predict the survival rates in prostate cancer patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable