S4306

Spirit blue agar

NutriSelect^® Plus, suitable for microbiology

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• final pH: 6.8±0.2 (25 °C)
• application(s): bioburden testing; cleaning products; cosmetics; food and beverages; personal care; microbiology
• suitability: lipolytic bacteria; nonselective and differential for Staphylococcus spp.

Description

Application

Used for the detection and enumeration of lipolytic microogranisms.

Components

Ingredients (g/L)
Casein enzymic hydrolysate, 10.00
Yeast extract, 5.00
Spirit blue, 0.15
Agar, 17.00

Preparation Note

Suspend 32.15 g of Spirit Blue Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and add slowly 30 ml of the lipase substrate while agitating to obtain an even distribution.
Note: To prepare the lipase substrate mix 1 ml of Tween 80 (Cat. No. 93780) in 400 ml warm distilled water. Add 100 ml of Cottonseed Oil (Cat. No. C7767) or Olive Oil (Cat. No. 75343). Agitate vigorously to emulsify.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves