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Key Documents

MR-107

Sigma-Aldrich

EmbryoMax® KSOM Mouse Embryo Media

(1X), Liquid, with 1/2 Amino Acids w/o BSA

Synonym(s):

Embryonic stem cell media

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71
Pricing and availability is not currently available.

Quality Level

300

form

liquid

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | embryo: suitable
cell culture | stem cell: suitable

input

sample type: mouse embryo(s)

Application

EmbryoMax® KSOM mouse embryo media has been used:
  • for culturing denuded mouse oocytes
  • in the washing step to perform mouse embryo collection
  • for culturing mouse zygotes

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Questions

  1. I would like to know the constitution of this medium. Thanks in advance.

    1 answer

    1. See below to review the formulation of this media:
      Catalog # MR-107 Working pH range 7.2 - 7.4
      Components (mg/L)
      CaCl2.2H2O 250.00
      KCL 186.38
      KH2PO4 47.99
      MgSO4.7H2O 49.30
      NaCl 5551.80
      NaHCO3 2100.25
      EDTA 3.72
      D-Glucose 36.03
      Sodium Lactate 1121.00
      Sodium Pyruvate 22.00
      BSA ---
      Phenol Red ---
      L-Arginine 63.20
      L-Cystine 12.02
      L-Glutamine 146.15
      Glycine 3.75
      L-Histidine.HCl.H2O 20.96
      L-Isoleucine 26.23
      L-Leucine 26.24
      L-Lysine.HCl 36.52
      L-Methionine 7.46
      L-Phenylalanine 16.52
      L-Serine 5.26
      L-Threonine 23.82
      L-Tryptophan 5.11
      L-Tyrosine 18.12
      L-Valine 23.42
      L-Alanine 4.45
      L-Asparagine-H2O 7.50
      L-Aspartic Acid 6.66
      L-Glutamic Acid 7.36
      L-Proline 5.76
      Pen G Na(units) 100,000
      Strep Sulfate 50.00

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